\documentclass[a4paper,12pt,pdftex]{exam} \newcommand{\ptitle}{Orientation tuning} \input{../header.tex} \firstpagefooter{Supervisor: Jan Benda}{phone: 29 74573}% {email: jan.benda@uni-tuebingen.de} \begin{document} \input{../instructions.tex} %%%%%%%%%%%%%% Questions %%%%%%%%%%%%%%%%%%%%%%%%% \begin{questions} \question In the visual cortex V1 orientation sensitive neurons respond to bars in dependence on their orientation. How is the orientation of a bar encoded by the activity of a population of orientation sensitive neurons? In an electrophysiological experiment, 6 neurons have been recorded simultaneously. First, the tuning of these neurons was characterized by presenting them bars in a range of 12 orientation angles. Each orientation was presented 50 times. Each of the \texttt{unit*.mat} files contains the responses of one of the neurons. In there, \texttt{angles} is a vector with the orientation angles of the bars in degrees. \texttt{spikes} is a cell array that contains the vectors of spike times for each angle and presentation. The spike times are given in seconds. The stimulation with the bar starts a time $t_0=0$ and ends at time $t_1=200$\,ms. Then the population activity of the 6 neurons was measured in response to arbitrarily oriented bars. The responses of the 6 neurons to 50 presentation of a bar are stored in the \texttt{spikes} variables of the \texttt{population*.mat} files. The \texttt{angle} variable holds the angle of the presented bar. \continue \begin{parts} \part Illustrate the spiking activity of the V1 cells in response to different orientation angles of the bars by means of spike raster plots (of a single unit). \part Plot the firing rate of each of the 6 neurons as a function of the orientation angle of the bar. As the firing rate compute the number of spikes in the time interval $0<t<200$\,ms divided by 200\,ms. The resulting curves are the tuning curves $r(\varphi)$ of the neurons. \part Fit the function \[ r(\varphi) = g \cdot (1+\cos(2(\varphi-\varphi_0)))/2 + a \] to the measured tuning curves in order to estimated the orientation angle at which the neurons respond strongest. In this function $\varphi_0$ is the position of the peak, $g$ is a gain factor that sets the modulation depth of the firing rate, and $a$ is an offset. \part How can the orientation angle of the presented bar be read out from one trial of the population activity of the 6 neurons? One possible method is the so called ``population vector'' where unit vectors pointing into the direction of the maximum response of each neuron are weighted by their firing rate. The stimulus orientation is then the direction of the averaged vectors. %Think of another (simpler) method how the orientation of the bar %may be approximately read out from the population. An alternative read out is maximum likelihood (see script). Load one of the \texttt{population*.mat} files, illustrate the data, and estimate the orientation angle of the bar from single trial data by the two different methods. \part Compare, illustrate and discuss the performance of the two decoding methods by using all of the recorded responses (all \texttt{population*.mat} files). How exactly is the orientation of the bar encoded? How robust is the estimate of the orientation from trial to trial? \end{parts} \end{questions} \end{document} gains and angles of the 6 neurons: gain=10.7 phase=5 gain=18.0 phase=38 gain=11.3 phase=71 gain=14.1 phase=108 gain=19.0 phase=138 gain=16.4 phase=174