LED_detect.py needs to be done for all recordings. next we need to check if trial_analysis.py does everything we need.
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@ -63,6 +63,7 @@ def main(file_path, check, x, y):
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LED_frames = np.arange(len(LED_val)-1)[(LED_val[:-1] < light_th) & (LED_val[1:] > light_th)]
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np.save(os.path.join(folder, 'LED_frames.npy'), LED_frames)
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fig, ax = plt.subplots()
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ax.plot(np.arange(len(LED_val)), LED_val, color='k')
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ax.plot(LED_frames, np.ones(len(LED_frames))*light_th, 'o', color='firebrick')
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@ -73,8 +74,8 @@ if __name__ == '__main__':
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parser = argparse.ArgumentParser(description='Detect frames of blinking LED in video recordings.')
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parser.add_argument('file', type=str, help='video file to be analyzed')
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parser.add_argument("-c", '--check', action="store_true", help="check if LED pos is correct")
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parser.add_argument('-x', type=int, nargs=2, default=[1240, 1250], help='x-borders of LED detect area (in pixels)')
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parser.add_argument('-y', type=int, nargs=2, default=[1504, 1526], help='y-borders of LED area (in pixels)')
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parser.add_argument('-x', type=int, nargs=2, default=[675, 695], help='x-borders of LED detect area (in pixels)')
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parser.add_argument('-y', type=int, nargs=2, default=[350, 360], help='y-borders of LED area (in pixels)')
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args = parser.parse_args()
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import glob
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@ -1,15 +1,78 @@
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import matplotlib.pyplot as plt
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import numpy as np
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import pandas as pd
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import os
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import sys
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import glob
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from IPython import embed
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def load_frame_times(trial_path):
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t_filepath = glob.glob(os.path.join(trial_path, '*.dat'))
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if len(t_filepath) == 0:
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return np.array([])
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else:
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t_filepath = t_filepath[0]
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f = open(t_filepath, 'r')
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frame_t = []
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for line in f.readlines():
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t = sum(x * float(t) for x, t in zip([3600, 60, 1], line.replace('\n', '').split(":")))
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frame_t.append(t)
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return np.array(frame_t)
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def load_and_converete_boris_events(trial_path, recording, sr, video_stated_FPS=25):
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def converte_video_frames_to_grid_idx(event_frames, led_frames, led_idx):
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event_idx_grid = (event_frames - led_frames[0]) / (led_frames[-1] - led_frames[0]) * (led_idx[-1] - led_idx[0]) + led_idx[0]
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return event_idx_grid
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# idx in grid-recording
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led_idx = pd.read_csv(os.path.join(trial_path, 'led_idxs.csv'), header=None).iloc[:, 0].to_numpy()
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# frames where LED gets switched on
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led_frames = np.load(os.path.join(trial_path, 'LED_frames.npy'))
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times, behavior, t_ag_on_off, t_contact = load_boris(trial_path, recording)
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contact_frame = np.array(np.round(t_contact * video_stated_FPS), dtype=int)
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ag_on_off_frame = np.array(np.round(t_ag_on_off * video_stated_FPS), dtype=int)
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# led_t_GRID = led_idx / sr
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contact_t_GRID = converte_video_frames_to_grid_idx(contact_frame, led_frames, led_idx) / sr
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ag_on_off_t_GRID = converte_video_frames_to_grid_idx(ag_on_off_frame, led_frames, led_idx) / sr
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return contact_t_GRID, ag_on_off_t_GRID, led_idx, led_frames
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def load_boris(trial_path, recording):
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boris_file = '-'.join(recording.split('-')[:3]) + '.csv'
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data = pd.read_csv(os.path.join(trial_path, boris_file))
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times = data['Start (s)']
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behavior = data['Behavior']
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t_ag_on = times[behavior == 0]
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t_ag_off = times[behavior == 1]
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t_ag_on_off = []
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for t in t_ag_on:
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t1 = np.array(t_ag_off)[t_ag_off > t]
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if len(t1) >= 1:
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t_ag_on_off.append(np.array([t, t1[0]]))
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t_contact = times[behavior == 2]
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return times, behavior, np.array(t_ag_on_off), t_contact.to_numpy()
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def main(data_folder=None):
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trials_meta = pd.read_csv('order_meta.csv')
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video_stated_FPS = 25. # cap.get(cv2.CAP_PROP_FPS)
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sr = 20_000
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for trial_idx in range(len(trials_meta)):
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group = trials_meta['group'][trial_idx]
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recording = trials_meta['recording'][trial_idx][1:-1]
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rec_id1 = trials_meta['rec_id1'][trial_idx]
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rec_id2 = trials_meta['rec_id2'][trial_idx]
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if group < 3:
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continue
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@ -21,18 +84,37 @@ def main(data_folder=None):
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if not os.path.exists(os.path.join(trial_path, 'led_idxs.csv')):
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continue
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print(group, recording)
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if not os.path.exists(os.path.join(trial_path, 'LED_frames.npy')):
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continue
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contact_t_GRID, ag_on_off_t_GRID, led_idx, led_frames = \
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load_and_converete_boris_events(trial_path, recording, sr)
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fund_v = np.load(os.path.join(trial_path, 'fund_v.npy'))
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ident_v = np.load(os.path.join(trial_path, 'ident_v.npy'))
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idx_v = np.load(os.path.join(trial_path, 'idx_v.npy'))
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times = np.load(os.path.join(trial_path, 'times.npy'))
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if len(uid:=np.unique(ident_v[~np.isnan(ident_v)])) >2:
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print(f'to many ids: {len(uid)}')
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print(f'ids in recording: {uid[0]:.0f} {uid[1]:.0f}')
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print(f'ids in meta: {rec_id1:.0f} {rec_id2:.0f}')
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fig, ax = plt.subplots(figsize=(30/2.54, 18/2.54))
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for id in uid:
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ax.plot(times[idx_v[ident_v == id]] / 3600, fund_v[ident_v == id], marker='.')
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ax.plot(contact_t_GRID / 3600, np.ones_like(contact_t_GRID) * 1050, '|', markersize=20, color='k')
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ax.plot(ag_on_off_t_GRID[:, 0] / 3600, np.ones_like(ag_on_off_t_GRID[:, 0]) * 1150, '|', markersize=20, color='red')
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ax.set_ylim(400, 1200)
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plt.show()
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LED_on_idx_DF = pd.read_csv(os.path.join(trial_path, 'led_idxs.csv'))
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i0 = np.array([int(LED_on_idx_DF.keys()[0])])
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LED_on_idx_DATA = np.concatenate((i0, np.array(LED_on_idx_DF).T[0]))
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LED_on_time_BORIS = np.load(os.path.join(trial_path, 'LED_on_time.npy'), allow_pickle=True)
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print(len(LED_on_idx_DATA), len(LED_on_time_BORIS))
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embed()
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quit()
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pass
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if __name__ == '__main__':
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main("/home/raab/data/mount_data/")
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# main("/home/raab/data/mount_data/")
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main("/home/raab/data/2020_competition_mount")
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