import numpy as np import os from Code.Functions.Utility import capacitance, stimulus_init, init_dict from Code.Functions.Cb_stellate_fxns import Cb_stellate_mut # model parameters dt = 0.01 sec = 2 low = 0 high = 0.0015 number_steps = 200 initial_period = 1000 num_gating = 9 num_current = 6 C, surf_area = capacitance(61.4, 1.50148) variable = np.array(['m', 'h', 'n', 'n_A', 'h_A', 'n_A_mut', 'h_A_mut', 'm_T', 'h_T']) stim_time, I_in, stim_num, V_m = stimulus_init(low, high, number_steps, initial_period, dt, sec) shift, scale, slope_shift, E, currents_included, b_param, g = init_dict(np.array(['m', 'h', 'n', 'n_A', 'h_A','n_A_mut', 'h_A_mut', 'm_T', 'h_T'])) V_init = -70 # initialize arrays current = np.zeros((num_current, stim_num)) gating = np.zeros((num_gating, stim_num)) # gating parameters b_param['m'][:] = np.array([-37., -3, 1]) b_param['h'] = np.zeros(4) b_param['h'][:] = np.array([-40., 4., 1., 0]) b_param['n'][:] = np.array([-23, -5, 1]) b_param['n_A'][:] = np.array([-27, -13.2, 1.]) b_param['h_A'][:] = np.array([-80., 6.5, 1.]) b_param['n_A_mut'][:] = np.array([-27, -13.2, 1.]) b_param['h_A_mut'][:] = np.array([-80., 6.5, 1.]) b_param['m_T'][:] = np.array([-50., -3, 1.]) b_param['h_T'][:] = np.array([-68., 3.75, 1.]) # reversal potentials E["Na"] = 55. E["K"] = -80. E["Ca"] = 22. E["Leak"] = -70. # as per Molineux et al 2005 and NOT the -38 in Alexander et al 2019 # model currents currents_included["Na"] = True currents_included["Kd"] = True currents_included["A_mut"] = True currents_included["A"] = True currents_included["T"] = True currents_included["Leak"] = True # model conductances g["Na"] = 3.4 * surf_area g["Kd"] = 9.0556 * surf_area g["A_mut"] = 15.0159 * surf_area * 0.5 g["A"] = 15.0159 * surf_area* 0.5 g["T"] = 0.45045 * surf_area g["Leak"] = 0.07407 * surf_area prominence = 50 min_spike_height = 0 desired_AUC_width = high/5 folder = '../Neuron_models' mut = 'Cb_stellate' mutations = {mut:{'g_ratio': 1, 'activation_Vhalf_diff': 0, 'activation_k_ratio':0}} if not os.path.isdir(folder): os.makedirs(folder) Cb_stellate_mut(V_init, g, E, I_in, dt, currents_included, stim_time, stim_num, C, shift, scale, b_param, slope_shift, gating, current, prominence, desired_AUC_width, mutations, mut, folder, high, low, number_steps, initial_period, sec)