101 lines
3.2 KiB
Python
101 lines
3.2 KiB
Python
import glob
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import pathlib
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import numpy as np
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import matplotlib.pyplot as plt
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import rlxnix as rlx
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from IPython import embed
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from scipy.signal import welch
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def binary_spikes(spike_times, duration, dt):
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"""
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Converts the spike times to a binary representations
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Parameters
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----------
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spike_times : np.array
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The spike times.
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duration : float
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The trial duration:
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dt : float
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The temporal resolution.
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Returns
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-------
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binary : np.array
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The binary representation of the spike train.
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"""
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binary = np.zeros(int(np.round(duration / dt))) #create the binary array with the same length as potential
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spike_indices = np.asarray(np.round(spike_times / dt), dtype = int) # get the indices
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binary[spike_indices] = 1 # put the indices into binary
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return binary
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def firing_rate(binary_spikes, dt = 0.000025, box_width = 0.01):
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box = np.ones(int(box_width // dt))
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box /= np.sum(box) * dt # normalisierung des box kernels to an integral of one
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rate = np.convolve(binary_spikes, box, mode = 'same')
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return rate
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def power_spectrum(rate, dt):
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freq, power = welch(rate, fs = 1/dt, nperseg = 2**16, noverlap = 2**15)
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return freq, power
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#find example data
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datafolder = "../data"
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example_file = datafolder + "/" + "2024-10-16-ad-invivo-1.nix"
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#load dataset
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dataset = rlx.Dataset(example_file)
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# find all sams
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sams = dataset.repro_runs('SAM')
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sam = sams[2] # our example sam
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potential,time = sam.trace_data("V-1") #membrane potential
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spike_times, _ = sam.trace_data('Spikes-1') #spike times
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df = sam.metadata['RePro-Info']['settings']['deltaf'][0][0] #find df in metadata
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amp = sam.metadata['RePro-Info']['settings']['contrast'][0][0] * 100 #find amplitude in metadata
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#figure for a quick plot
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fig = plt.figure(figsize = (5, 2.5))
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ax = fig.add_subplot()
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ax.plot(time[time < 0.1], potential[time < 0.1]) # plot the membrane potential in 0.1s
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ax.scatter(spike_times[spike_times < 0.1],
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np.ones_like(spike_times[spike_times < 0.1]) * np.max(potential)) #plot teh spike times on top
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plt.show()
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plt.close()
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# get all the stimuli
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stims = sam.stimuli
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# empty list for the spike times
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spikes = []
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#spikes2 = np.array(range(len(stims)))
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# loop over the stimuli
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for stim in stims:
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# get the spike times
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spike, _ = stim.trace_data('Spikes-1')
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# append the first 100ms to spikes
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spikes.append(spike[spike < 0.1])
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# get stimulus duration
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duration = stim.duration
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ti = stim.trace_info("V-1")
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dt = ti.sampling_interval # get the stimulus interval
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bin_spikes = binary_spikes(spike, duration, dt) #binarize the spike_times
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print(len(bin_spikes))
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pot,tim= stim.trace_data("V-1") #membrane potential
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rate = firing_rate(bin_spikes, dt = dt)
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print(np.mean(rate))
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fig, [ax1, ax2] = plt.subplots(1, 2,layout = 'constrained')
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ax1.plot(tim,rate)
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ax1.set_ylim(0,600)
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ax1.set_xlim(0, 0.04)
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freq, power = power_spectrum(rate, dt)
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ax2.plot(freq,power)
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ax2.set_xlim(0,1000)
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# make an eventplot
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fig = plt.figure(figsize = (5, 3), layout = 'constrained')
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ax = fig.add_subplot()
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ax.eventplot(spikes, linelength = 0.8)
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ax.set_xlabel('time [ms]')
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ax.set_ylabel('loop no.')
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plt.show() |