import matplotlib.pyplot as plt import numpy as np from read_chirp_data import * from read_baseline_data import * from utility import * from IPython import embed # define data path and important parameters data_dir = "../data" sampling_rate = 40 #kHz cut_window = 100 cut_range = np.arange(-cut_window * sampling_rate, 0, 1) window = 1 inch_factor = 2.54 datasets = ["2018-11-13-ad-invivo-1", "2018-11-13-aj-invivo-1", \ "2018-11-13-ak-invivo-1", "2018-11-14-ad-invivo-1"] # dataset = "2018-11-20-af-invivo-1" fig = plt.figure() # figsize=(5 / inch_factor, 2.5 / inch_factor)) fig.set_size_inches((35/inch_factor, 15/inch_factor)) axes = [] axes.append(fig.add_subplot(221)) axes.append(fig.add_subplot(222)) axes.append(fig.add_subplot(223)) axes.append(fig.add_subplot(224)) for dataset, ax in zip(datasets, axes): base_spikes = read_baseline_spikes(os.path.join(data_dir, dataset)) base_spikes = base_spikes[1000:2000] spikerate = len(base_spikes) / base_spikes[-1] print(spikerate) # read spikes during chirp stimulation spikes = read_chirp_spikes(os.path.join(data_dir, dataset)) df_map = map_keys(spikes) rates = {} # iterate over df for deltaf in df_map.keys(): rates[deltaf] = {} beat_duration = int(abs(1 / deltaf) * 1000) beat_window = 0 while beat_window + beat_duration <= cut_window/2: beat_window = beat_window + beat_duration for x, repetition in enumerate(df_map[deltaf]): for phase in spikes[repetition]: # get spikes some ms before the chirp first chirp spikes_to_cut = np.asarray(spikes[repetition][phase]) spikes_cut = spikes_to_cut[(spikes_to_cut > -cut_window) & (spikes_to_cut < 0)] spikes_idx = np.round(spikes_cut * sampling_rate) # also save as binary, 0 no spike, 1 spike binary_spikes = np.isin(cut_range, spikes_idx) * 1 smoothed_data = smooth(binary_spikes, window, 1 / sampling_rate) modulation = np.std(smoothed_data) rates[deltaf][x] = modulation break for i, df in enumerate(sorted(rates.keys())): max_rep = len(sorted(rates[df].keys()))-1 for j, rep in enumerate(rates[df].keys()): if j == max_rep: farbe = 'royalblue' gro = 8 else: farbe = 'k' gro = 6 ax.plot(df, rates[df][rep], marker='o', color=farbe, ms=gro) ax.set_xlim(-450, 800) ax.set_ylim(0.1, 0.3) ax.set_xticks(np.arange(-400, 810, 100)) ax.yaxis.set_tick_params(labelsize=18) ax.xaxis.set_tick_params(labelsize=18) ax.spines["top"].set_visible(False) ax.spines["right"].set_visible(False) axes[0].set_ylabel('Firing rate modulation', fontsize=22) axes[0].yaxis.set_label_coords(-0.15, -.125) axes[2].set_xlabel('$\Delta$f [Hz]', fontsize=22) axes[3].set_xlabel('$\Delta$f [Hz]', fontsize=22) axes[1].set_yticklabels([]) axes[3].set_yticklabels([]) axes[0].set_xticklabels([]) axes[1].set_xticklabels([]) axes[2].set_xticklabels(['-400', '', '-200', '', '0', '', '200', '', '400', '', '600', '', '800'], rotation=45) axes[3].set_xticklabels(['-400', '', '-200', '', '0', '', '200', '', '400', '', '600', '', '800'], rotation=45) fig.subplots_adjust(left=0.09, bottom=0.175, right=0.975, top=0.95) fig.savefig('spikes_beat_20af.png')